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Harlequin E.coli/Coliform Medium

Product Code: HAL008

A dual chromogenic medium for the simultaneous enumeration of Escherichia coli and coliforms in food and environmental samples.

Application(s): Industrial, Food, Dairy
Organism(s): Enterobacteriaceae / Coliforms, E.coli

The different colony types are simple to distinguish allowing rapid counting of both E.coli and coliforms on a single medium.

Based upon the formulation of Tryptone Bile Agar LAB072, the medium has been modified by the addition of two chromogenic substrates, one to detect the ß-glucuronidase enzyme (X-glucuronide) and another to detect the ß-galactosidase enzyme (magenta-ß-gal). Typical E.coli strains possess both enzymes but only cleave the X-glucuronide substrate, thereby producing blue-green colonies. Typical coliforms, however, possess only the ß-galactosidase enzyme and produce rose-pink colonies.

The colony types are easily distinguishable, even in the presence of other organisms, or when large numbers are observed, making simultaneous enumeration of E.coli and coliforms a quick and simple procedure.